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Cell Biology
Inhibitors
Protease/Phosphatase Inhibitor Cocktail (100X)
Protease/Phosphatase Inhibitor Cocktail (100X) from Cell Signaling Technology
Be the first to write a review!
Citations:
(432)
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Cell Signaling Technology for
Protease/Phosphatase Inhibitor Cocktail (100X)
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Company Info
Citations
Reviews
Product Specs
Item
Protease/Phosphatase Inhibitor Cocktail (100X)
Company
Cell Signaling Technology
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
5872S
Quantity
1 ml
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Cell Signaling Technology
3 Trask Lane
Danvers
,
Massachusetts
01923
United States
Phone
: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488
Company Profile
Website:
http://www.cellsignal.com/
Website:
Technical Support
Citations (432)
(41)
AKAP13 Enhances CREB1 Activation by FSH in Granulosa Cells
Reproductive Sciences
May 1, 2023
Kamaria C. Cayton Cayton Vaught, Dana Hazimeh, Ashlie Sewdass Carter, Kate Devine, Jacqueline Y. Maher, Marcy Maguire, Elizabeth A. McGee, Paul H. Driggers, James H. Segars
(Pierce,
Thermo Scientific
,
87787
) with protease and phosphatase
inhibitors
(
Cell Signaling Technology
,
5872
) and lysates formed. Protein concentration was determined as above. An affinity-purified polyclonal
(42)
Spatial transcriptomic analysis of Sonic hedgehog medulloblastoma identifies that the loss of heterogeneity and promotion of differentiation underlies the response to CDK4/6 inhibition
Genome medicine
May 1, 2023
Vo T, Balderson B, Jones K, Ni G, Crawford J, Millar A, Tolson E, Singleton M, Kojic M, Robertson T,
et al
.
sodium deoxycholate, 0.1% SDS) with protease and phosphatase
inhibitors
(
Cell Signalling
Technology,
5872
) and 1µL/mL Benzonase® nuclease (Millipore, E1014). Total protein concentrations were determined using
(43)
P2X7 receptor contributes to long-term neuroinflammation and cognitive impairment in sepsis-surviving mice
Frontiers in Pharmacology
April 21, 2023
Vinícius Santos Alves, Joyce Pereira da da Silva, Fabiana Cristina Rodrigues, Suzana Maria Bernardino Araújo, André Luiz Gouvêa, Raíssa Leite-Aguiar, Stephanie Alexia Cristina Silva Santos, Milla Souza Pessoa da da Silva, Fernanda Silva Ferreira, Eduardo Peil Marques,
et al.
with Protease/Phosphatase inhibitor cocktail (
Cell Signaling
, Danvers, Massachusetts, United States; #
5872S
). The lysates were centrifuged at 14,000 × g for 30 min at 4°C. The supernatant was transferred into
(44)
Intestinal transit amplifying cells require METTL3 for growth factor signaling, KRAS expression, and cell survival
bioRxiv : the preprint server for biology
April 21, 2023
Danan CH, Naughton KE, Hayer KE, Vellappan S, McMillan EA, Zhou Y, Matsuda R, Nettleford SK, Katada K, Parham LR,
et al
.
were lysed using RIPA buffer (
CST
9806S
) supplemented with
1:100
protease phosphatase
inhibitor
(
CST
5872S
) and protein concentration was measured using the
Pierce™ BCA Protein Assay Kit
(
Thermo
23225
). Lysates
(45)
Syngeneic mouse model of human HER2+ metastatic breast cancer for the evaluation of trastuzumab emtansine combined with oncolytic rhabdovirus
Frontiers in Immunology
April 19, 2023
Zaid Taha, Mathieu J.F. Crupi, Nouf Alluqmani, Faiha Fareez, Kristy Ng, Judy Sobh, Emily Lee, Andrew Chen, Max Thomson, Marcus M. Spinelli,
et al.
Na2P2O7, protease/phosphatase
inhibitor
cocktail (Cell Signaling Technology (
CST
), MA, USA, Cat. #
5872
) and 1% Triton X-100 on ice. Protein concentration was determined by bicinchoninic acid
assay
(
Thermo Scientific
(46)
Obesity partially potentiates dimethylbenz[a]anthracene-exposed ovotoxicity by altering the DNA damage repair response in mice†
Biology of reproduction
April 11, 2023
Rishi JK, Timme K, White HE, Kerns KC, Keating AF.
USA). Protease/phosphatase
inhibitor
cocktail was purchased from
Cell Signaling Technology
(catalog #
5872
; Danvers, MA, USA). Animal exposure and tissue collection Female C57Bl6 (KK.Cg-a/a; n = 20) wild-type
(47)
MLKL deficiency protects against low-grade, sterile inflammation in aged mice
Cell Death & Differentiation
April 1, 2023
Emma C. Tovey Crutchfield, Sarah E. Garnish, Jessica Day, Holly Anderton, Shene Chiou, Anne Hempel, Cathrine Hall, Komal M. Patel, Pradnya Gangatirkar, Katherine R. Martin,
et al.
RIPA buffer supplemented with Protease & Phosphatase Inhibitor Cocktail (
Cell Signaling Technology
5872
S, 50 mg/ml) and Benzonase (Sigma-Aldrich E1014, 100 U/mL). Tissue lysates were homogenized using the
(48)
PSMD2 contributes to the progression of esophageal squamous cell carcinoma by repressing autophagy
Cell & bioscience
March 30, 2023
Liu Y, Wu M, Xu S, Niu X, Liu W, Miao C, Lin A, Xu Y, Yu L.
lentivirus
were harvested in sample buffer supplemented with Protease/Phosphatase Inhibitor Cocktail (#
5872
,
Cell signaling Technology
, Shanghai, China). Protein concentrations were detected by bicinchoninic
(49)
Sepsis-like Energy Deficit Is Not Sufficient to Induce Early Muscle Fiber Atrophy and Mitochondrial Dysfunction in a Murine Sepsis Model
Biology (Basel)
March 30, 2023
Alexandre Pierre, Claire Bourel, Raphael Favory, Benoit Brassart, Frederic Wallet, Frederic N. Daussin, Sylvain Normandin, Michael Howsam, Raphael Romien, Jeremy Lemaire,
et al.
1% Triton) with 1 mM phenylmethanesulfonyl fluoride and a protease/phosphatase
inhibitor
cocktail (#
5872
,
Cell Signaling
, Danvers, MA, USA) using a Bead Mill 4
Homogenizer
(
Thermo Fisher Scientific
, Waltham,
(50)
Downregulation of BUD31 Promotes Prostate Cancer Cell Proliferation and Migration via Activation of p-AKT and Vimentin In Vitro
International journal of molecular sciences
March 23, 2023
Choudhry M, Gamallat Y, Khosh Kish E, Seyedi S, Gotto G, Ghosh S, Bismar TA.
#
9806
,
Cell Signaling
, Danvers, MA, USA) with the addition of
1:100
protease
PMSF
inhibitors
(Cat #
5872S
,
Cell Signaling
, Danvers, MA, USA). When running the
Western blot
, equal quantities of proteins were
(51)
Grpel2 maintains cardiomyocyte survival in diabetic cardiomyopathy through DLST-mediated mitochondrial dysfunction: a proof-of-concept study
Journal of translational medicine
March 16, 2023
Yang R, Zhang X, Zhang Y, Wang Y, Li M, Meng Y, Wang J, Wen X, Yu J, Chang P.
87787
,
Thermo Fisher Scientific
, USA) supplemented with a protease and phosphatase
inhibitor
cocktail (
5872
,
CST
, USA). Subsequent
procedures
were performed as previously described [27]. The homogenates were
(52)
The Potent G-Quadruplex-Binding Compound QN-302 Downregulates S100P Gene Expression in Cells and in an In Vivo Model of Pancreatic Cancer
Molecules
March 7, 2023
Ahmed A. Ahmed, William Greenhalf, Daniel H. Palmer, Nicole Williams, Jenny Worthington, Tariq Arshad, Shozeb Haider, Effrosyni Alexandrou, Dilek Guneri, Zoe A. E. Waller,
et al.
(radio-
immunoprecipitation assay
) buffer (
CST
#
9806
) supplemented with phosphatase and
protease inhibitors
(
CST
#
5872
) and PMSF protease inhibitor (
CST
#
8553
). Lysate was isolated and total protein quantified for each sample
(53)
Surfactant protein A alters endosomal trafficking of influenza A virus in macrophages
Frontiers in Immunology
March 7, 2023
Eric Yau, Linlin Yang, Yan Chen, Todd M. Umstead, Hannah Atkins, Zoe E. Katz, Jonathan W. Yewdell, Chintan K. Gandhi, E. Scott Halstead, Zissis C. Chroneos
Tris-HCL pH 8.1, 10mM EDTA pH 8.0) with 1x Protease/Phosphatase Inhibitor Cocktail (
Cell Signaling
Cat No
5872S
). Samples were thawed and sonicated at 50% amplitude for 10 seconds in 2 second ON/OFF interval using
(54)
Structural studies identify angiotensin II receptor blocker-like compounds as branched-chain ketoacid dehydrogenase kinase inhibitors
Journal of Biological Chemistry
March 1, 2023
Shenping Liu, Bethany L. Kormos, John D. Knafels, Parag V. Sahasrabudhe, Amy Rosado, Ruth F. Sommese, Allan R. Reyes, Jessica Ward, Rachel J. Roth Flach, Xiaochun Wang,
et al.
biotinylated total BCKD antibody and 1× protease/phosphatase
inhibitor
cocktail (
Cell Signaling
; catalog no.:
5872
). After an hour of
incubation
at room temperature, a 1×
immunoassay
buffer (
PerkinElmer
; catalog no.:
(55)
A TREM2-activating antibody with a blood-brain barrier transport vehicle enhances microglial metabolism in Alzheimer's disease models
Nature neuroscience
March 1, 2023
van Lengerich B, Zhan L, Xia D, Chan D, Joy D, Park JI, Tatarakis D, Calvert M, Hummel S, Lianoglou S,
et al
.
buffer (
Thermo Fisher Scientific
,
87787
) containing 1× protease
inhibitor
(
Cell Signaling Technology
,
5872
) using a cell scraper. Cell lysate was normalized based on BCA measurement. Then, 200–400 µg of total
(56)
Development of Human Carbonic Anhydrase II Heterobifunctional Degraders
Journal of Medicinal Chemistry
February 23, 2023
Conor B. O’Herin, Yuta W. Moriuchi, Troy A. Bemis, Alysia J. Kohlbrand, Michael D. Burkart, Seth M. Cohen
cell lysis
buffer (
Cell Signaling Technology
,
9803
), protease
inhibitor
cocktail (
Cell Signaling Technology
,
5872
), and 1 mM phenylmethylsulfonylfluoride, was added to each well, and the plates were rocked at 4 °C
(57)
Impact of Acute High Glucose on Mitochondrial Function in a Model of Endothelial Cells: Role of PDGF-C
International Journal of Molecular Sciences
February 23, 2023
Adriana Grismaldo Rodríguez, Jairo Zamudio Rodríguez, Alfonso Barreto, Sandra Sanabria-Barrera, José Iglesias, Ludis Morales
Thermo Fisher Scientific
/Invitrogen (Chelmsford, MA, USA). Protease and phosphatase inhibitor cocktail (
5872
), antibodies against OPA1 (D7C1A), MFN1 (D6E2S), MFN2 (D1E9), DRP1 (D6C7), DRP1pS616, β actin (8H10D10),
(58)
Glycyl-tRNA Synthetase (GARS) Expression Is Associated with Prostate Cancer Progression and Its Inhibition Decreases Migration, and Invasion In Vitro
International Journal of Molecular Sciences
February 21, 2023
Ealia Khosh Kish, Yaser Gamallat, Muhammad Choudhry, Sunita Ghosh, Sima Seyedi, Tarek A. Bismar
using
RIPA buffer
(
Sigma-Aldrich
, St. Louis, MO, USA) pre-mixed with
protease inhibitors
and
PMSF
(Cat#
5872S
,
Cell signaling
, Danvers, MA, USA). When loading on the polyacrylamide SDS gel, equal quantities of
(59)
Lacrimal Gland Epithelial Cells Shape Immune Responses through the Modulation of Inflammasomes and Lipid Metabolism
International journal of molecular sciences
February 21, 2023
Delcroix V, Mauduit O, Yang M, Srivastava A, Umazume T, de Paiva CS, Shestopalov VI, Dartt DA, Makarenkova HP.
Tris-HCl + 150 mM NaCl + 1 mM EGTA) and supplemented with protease/phosphatase
inhibitor
cocktail (#
5872
,
Cell Signaling
, Danvers, MA, USA). The tissue was homogenized with the Omni Bead Ruptor 4 (#
25-010
,
(60)
Selenium Protects Mouse Hypothalamic Cells from Glucocorticoid-Induced Endoplasmic Reticulum Stress Vulnerability and Insulin Signaling Impairment
Antioxidants (Basel)
February 20, 2023
Katlyn J. An, Ashley N. Hanato, Katherine W. Hui, Matthew W. Pitts, Lucia A. Seale, Jessica L. Nicholson, Pamela Toh, Jun Kyoung Kim, Marla J. Berry, Daniel J. Torres
Tissue Buffer (
Millipore Sigma
, #
C3228
) containing a protease/phosphatase
inhibitor
(
Cell Signaling
, #
5872
), centrifuged, and the supernatant collected. Proteins (80 µg per sample) were loaded onto 4–20% gradient
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Reviews
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Protease/Phosphatase Inhibitor Cocktail (100X) from Cell Signaling Technology
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
Citations
Reviews
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